Artículos de revistas
An HPLC method for determination of azadirachtin residues in bovine muscle
Fecha
2011Registro en:
Journal of Chromatographic Science, Volumen 49, Issue 4, 2011, Pages 327-331
00219665
10.1093/chrsci/49.4.327
Autor
Gai, María Nella
Álvarez, Christian
Venegas, Raúl
Morales, Javier
Institución
Resumen
A high-performance liquid chromatography (HPLC) method for the determination of azadirachtin (A and B) residues in bovine muscle has been developed. Azadirachtin is a neutral triterpene and chemotherapeutic agent effective in controlling some pest flies in horses, stables, horns and fruit. The actual HPLC method uses an isocratic elution and UV detection. Liquid-liquid extraction and solid-phase purification was used for the clean-up of the biological matrix. The chromatographic determination of these components is achieved using a C18 analytical column with water-acetonitrile mixture (27.5:72.5, v/v) as mobile phase, 1 mL/min as flow rate, 45 °C column temperature and UV detector at 215 nm. The azadirachtin peaks are well resolved and free of interference from matrix components. The extraction and analytical method developed in this work allows the quantitation of azadirachtin with precision and accuracy, establishing a lower limit of quantitation of azadirachtin, extracted from the biological matrix.