Whole-genome comparative analysis of the pathogen Piscirickettsia salmonis

Abstract

The intracellular pathogen Piscirickettsia salmonis is the etiological agent of piscirickettsiosis, the most important bacterial disease that affects the Chilean salmon industry. Despite its importance, little is known regarding the biology of the pathogen. In this study, recently published sequencing data was used in order to characterize the genome of P. salmonis, defining groups of genes associated with bacterial processes such as, invasion and intracellular survival. Moreover, one Chilean P. salmonis isolate, which is known to be virulent at in vitro and in vivo assays, was sequenced, assembled, annotated and functionally characterized. Whole-genome comparisons between public P. salmonis isolates confirmed the existence of two different genogroups associated with the LF-89 and EM-90 strains, and the bacterial pan and core genome were defined. Additionally, differences were observed at the genomic level between the P. salmonis reference strain and a Norwegian isolate, which is known to produce milder piscirickettsiosis outbreaks. Finally, candidate genes for invasion and intracellular survival were chosen from phylogenetically related bacteria, and annotated in P. salmonis using comparative genomics. These results showed the presence of several genes that might be related to bacterial pathogenesis, for example those of the type III, IV and VI secretion systems, in which some amino acidic differences within both genogroups and the Norwegian isolate were established. Altogether, these results will be relevant for understanding the host-pathogen interaction and further studies, aimed at generating new disease control strategies, should be devised using this information.