Artículos de revistas
Detección de isoenzimas sintasas de óxido nítrico (NOS) en membrana sinovial de articulaciones metacarpofalángicas equinas normales y con daño macroscópico
Fecha
2011Registro en:
Arch Med Vet 43, 181-185 (2011)
0301732X
07176201
10.4067/S0301-732X2011000200012
Autor
Galleguillos, M.
Núñez, F.
Letelier, M. E.
Contesse, B.
Adarmes Ahumada, Héctor
Institución
Resumen
We studied the presence of NOS isoforms in synovial membrane from equine metacarpophalangeal joint. This was considered a normal joint group
NN (n = 13) when there was not any visual damage of joint structures. The damaged groups were classified as follows: NA (n = 8) with congestive
synovial membrane without cartilage damage; AN (n = 10) cartilage damage and normal synovial membrane and AA (n = 8) those with cartilage
damage and congestive synovial membrane. Damaged cartilage showed erosions and multiple wear lines. Detection of NOS isoforms was performed
by western blot using monoclonal antibodies. The generated chemiluminescence was measured and expressed in densitometric relative units (DRU)
that represent the rate between the pixels of each sample and those of the standard. NOS I showed a 145 kDa band in all samples, without significant
differences between joint groups. NOS III showed a 140 kDa band, with detection percentages of NN = 77%; NA = 63%; AN = 30% and AA = 100%,
without significant differences between them. NOS II was no detected in these samples. In conclusion, NOS I and partially NOS III, were expressed in
a constitutive form in equine synovial membrane, while NOS II was not detected in these assays, which was likely to be expected given the absence of
acute inflammation, at least during macroscopic observation.