Identification of sex chromosome molecular markers using RAPDs and fluorescent in situ hybridization in rainbow trout

dc.creatorIturra Constant, Iris
dc.creatorMedrano, J. F.
dc.creatorBagley, M.
dc.creatorLam Pastén, Natalia
dc.creatorVergara Proboste, Nicolás
dc.creatorMarín, J. C.
dc.date.accessioned2018-12-20T14:52:34Z
dc.date.available2018-12-20T14:52:34Z
dc.date.created2018-12-20T14:52:34Z
dc.date.issued1997
dc.identifierGenetica, Volumen 101, Issue 3, 2018, Pages 209-213
dc.identifier00166707
dc.identifier10.1023/A:1018371623919
dc.identifierhttp://repositorio.uchile.cl/handle/2250/157122
dc.description.abstractThe goal of this work is to identify molecular markers associated with the sex chromosomes in rainbow trout to study the mode of sex determination mechanisms in this species. Using the RAPD assay and bulked segregant analysis, two markers were identified that generated polymorphic bands amplifying preferentially in males of the Mount Lassen and Scottish strains of rainbow trout. Chromosomal localization using fluorescent in situ hybridization of a 900 bp probe developed from one of these markers revealed a brightly defined signal on a chromosome that could morphologically be classified as the Y chromosome.
dc.languageen
dc.publisherKluwer Academic Publishers
dc.sourceGenetica
dc.subjectFish
dc.subjectRainbow trout
dc.subjectRAPD
dc.subjectSex chromosome markers
dc.titleIdentification of sex chromosome molecular markers using RAPDs and fluorescent in situ hybridization in rainbow trout
dc.typeArtículos de revistas


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