Artículos de revistas
Identification and structural analysis of a ribosomal RNA gene promoter from Thiobacillus ferrooxidans
Fecha
1990Registro en:
FEBS Letters, Volumen 272, Issue 1-2, 2018, Pages 50-54
00145793
10.1016/0014-5793(90)80446-P
Autor
Takamiya, Monica
Salazar, Oriana
Vargas, Danilo
Jedlicki, Eugenia
Orellana, Omar
Institución
Resumen
The 5'-terminus of a rRNA operon (rrnT2) from Thiobacillus ferrooxidans was characterized. The rRNA promoters from this microorganism were identified by means of a functional assay in Escherichia coli. DNA sequencing of the promoter region, upstream the 16 S rRNA gene, showed the presence of a consensus sequence for bacterial ribosomal promoters. Other features such as a 'discriminator' sequence, antiterminator elements and an upstream hexanucleotide common to several rRNA operons were also found. Two other putative transcription promoters were also identified. © 1990.