Artículos de revistas
Mouse sperm patch-clamp recordings reveal single Cl- channels sensitive to niflumic acid, a blocker of the sperm acrosome reaction
Fecha
1998Registro en:
FEBS Letters, Volumen 426, Issue 1, 2018, Pages 47-51
00145793
10.1016/S0014-5793(98)00305-6
Autor
Espinosa, F.
De La Vega-Beltrán, J. L.
López-González, I.
Delgado, R.
Labarca, P.
Darszon, A.
Institución
Resumen
Ion channels lie at the heart of gamete signaling. Understanding their regulation will improve our knowledge of sperm physiology, and may lead to novel contraceptive strategies. Sperm are tiny (~ 3 μm diameter) and, until now, direct evidence of ion channel activity in these cells was lacking. Using patch-clamp recording we document here, for the first time, the presence of cationic and anionic channels in mouse sperm. Anion selective channels were blocked by niflumic acid (NA) (IC50 = 11 μM). The blocker was effective also in inhibiting the acrosome reaction induced by the zona pellucida, GABA or progesterone. These observations suggest that Cl- channels participate in the sperm acrosome reaction in mammals.