Artículos de revistas
An inactive mutant of the α subunit of protein kinuse CK2 that traps the regulatory CK2β subunit
Fecha
1997Registro en:
FEBS Letters, Volumen 410, Issue 2-3, 2018, Pages 391-396
00145793
10.1016/S0014-5793(97)00625-X
Autor
Cosmelli, Diego
Antonelli, Marcelo
Allende, Catherine C.
Allende, Jorge E.
Institución
Resumen
Protein kinase CK2 (casein kinase 2) is a ubiquitous Ser/Thr protein kinase involved in cell proliferation. Mutation of the α subunit of the Xenopus laevis CK2 to change aspartic acid 156 to alanine (CK2αA156) resulted in an inactive enzyme. The CK2αA156 mutant, however, binds the regulatory subunit as measured by retention of β on a nickel chelating column mediated by (His)6-tagged CK2αA156. Addition of CK2αA156 also caused β to shift sedimentation in a sucrose gradient from a (β2) dimer (52 kDa) to an (α2β2) tetramer (130000 kDa). CK2αA156 can trap the β subunit in an inactive complex reducing the stimulation of casein phosphorylation caused by addition of β to wild-type α. This competitive effect depends on the ratio of α/αA156 and on the amount of β available. Since β inhibits the phosphorylation of calmodulin by CK2α, the addition of CK2αA156, in this case, increases calmodulin phosphorylation by the a and β combination. These results suggest that CK2αA156 may be a useful dominant