Artículo de revista
Pacific ciguatoxin-1b effect over Na + and K + currents, inositol 1,4,5-triphosphate content and intracellular Ca 2+ signals in cultured rat myotubes
Fecha
2002Registro en:
British Journal of Pharmacology, Volumen 137, Issue 7, 2018, Pages 1055-1062
00071188
10.1038/sj.bjp.0704980
Autor
Hidalgo, Jorge
Liberona, José Luis
Molgó, Jordi
Jaimovich Pérez, Enrique
Institución
Resumen
1. The action of the main ciguatoxin involved in ciguatera fish poisoning in the Pacific region (P-CTX-1b) was studied in myotubes originated from rat skeletal muscle cells kept in primary culture. 2. The effect of P-CTX-1b on sodium currents at short times of exposure (up to 1 min) showed a moderate increase in peak Na + current. During prolonged exposures, P-CTX-1b decreased the peak Na + current. This action was always accompanied by an increase of leakage currents, tail currents and outward Na + currents, resulting in an intracellular Na + accumulation. This effect is blocked by prior exposure to tetrodotoxin (TTX) and becomes evident only after washout of TTX. 3. Low to moderate concentrations of P-CTX-1b (2-5 nM) partially blocked potassium currents in a manner that was dependent on the membrane potential. 4. P-CTX-1b (2-12 nM) caused a small membrane depolarization (3-5 mV) and an increase in the frequency of spontaneous action potential discharges that reached in general low fr