Artículos de revistas
Carotenoid production and gene expression in an astaxanthin-overproducing Xanthophyllomyces dendrorhous mutant strain
Fecha
2015Registro en:
Archives of Microbiology, Volumen 197, Issue 10, 2018, Pages 1129-1139
1432072X
03028933
10.1007/s00203-015-1153-9
Autor
Castelblanco Matiz, Lina
Barbachano Torres, Alejandra
Ponce Noyola, Teresa
Ramos Valdivia, Ana
Cerda García‑Rojas, Carlos
Flores Ortiz, César
Barahona Crisóstomo, Salvador
Baeza Cancino, Marcelo
Alcaíno Gorman, Jennifer
Cifuentes Guzmán, Víctor
Institución
Resumen
© 2015, Springer-Verlag Berlin Heidelberg. The primary carotenoid synthesized by Xanthophyllomycesdendrorhous is astaxanthin, which is used as a feed additive in aquaculture. Cell growth kinetics and carotenoid production were correlated with the mRNA levels of the idi, crtE, crtYB, crtI, crtS and crtR genes, and the changes in gene sequence between the wild-type and a carotenoid overproducer XR4 mutant strain were identified. At the late stationary phase, the total carotenoid content in XR4 was fivefold higher than that of the wild-type strain. Additionally, the mRNA levels of crtE and crtS increased during the XR4 growth and were three times higher than the wild-type strain in the late stationary phase. Moreover, the nucleotide sequences of crtYB, crtI and crtR exhibited differences between the strains. Both the higher crtE and crtS transcript levels and the crtYB, crtI and crtR mutations can, at least in part, act to up-regulate the carotenoid biosynthesis pathway in the XR4 strain.