Artículo de revista
Dual effect of serotonin on the dendritic growth of cultured hippocampal neurons: Involvement of 5-HT1A and 5-HT7 receptors
Fecha
2017Registro en:
Molecular and Cellular Neuroscience, Volumen 85,
10959327
10447431
10.1016/j.mcn.2017.09.009
Autor
Rojas, P. S.
Aguayo, F.
Neira, D.
Tejos, M.
Aliaga, E.
Muñoz, J. P.
Parra, C. S.
Fiedler Temer, Jenny
Institución
Resumen
© 2017 Elsevier Inc. Serotonin acts through its receptors (5-HTRs) to shape brain networks during development and modulates essential functions in mature brain. The 5-HT1AR is mainly located at soma of hippocampal neurons early during brain development and its expression gradually shifts to dendrites during postnatal development. The 5-HT7R expressed early during hippocampus development, shows a progressive reduction in its expression postnatally. Considering these changes during development, we evaluated in cultured hippocampal neurons whether the 5-HT1AR and 5-HT7R change their expression, modulate dendritic growth, and activate signaling pathways such as ERK1/2, AKT/GSK3β and LIMK/cofilin, which may sustain dendrite outgrowth by controlling cytoskeleton dynamics. We show that mRNA levels of both receptors increase between 2 and 7 DIV; however only protein levels of 5-HT7R increase significantly at 7 DIV. The 5-HT1AR is preferentially distributed in the soma, while 5-HT7R displays a