Artículos de revistas
Global transcriptomic analysis uncovers a switch to anaerobic metabolism in tellurite-exposed Escherichia coli
Fecha
2014Registro en:
Research in Microbiology, Volumen 165, Issue 7, 2018, Pages 566-570
17697123
09232508
10.1016/j.resmic.2014.07.003
Autor
Molina Quiroz, Roberto
Loyola, David E.
Díaz Vásquez, Waldo A.
Arenas, Felipe A.
Urzúa Tobar, Ulises
Pérez Donoso, José
Vásquez, Claudio C.
Institución
Resumen
© 2014 Institut Pasteur. Tellurite (TeO32-) is harmful for most microorganisms, especially Gram-negative bacteria. Even though tellurite toxicity involves a number of individual aspects, including oxidative stress, malfunctioning of metabolic enzymes and a drop in the reduced thiol pool, among others, the general mechanism of toxicity is rather complex and not completely understood to date. This work focused on DNA microarray analysis to evaluate the Escherichia coli global transcriptomic response when exposed to the toxicant.Confirming previous results, the induction of the oxidative stress response regulator soxS was observed. Upregulation of a number of genes involved in the global stress response, protein folding, redox processes and cell wall organization was also detected. In addition, downregulation of aerobic respiration-related genes suggested a metabolic switch to anaerobic respiration. The expression results were validated through oxygen consumption experiments, which corrob