Artículos de revistas
Adrenalectomy promotes a permanent decrease of plasma corticoid levels and a transient increase of apoptosis and the expression of Transforming Growth Factor β1 (TGF-β1) in hippocampus: Effect of a TGF-β1 oligo-antisense
Fecha
2006Registro en:
BMC Neuroscience, Volumen 7,
14712202
14712202
10.1186/1471-2202-7-40
Autor
Bravo, Javier A.
Parra, Claudio S.
Arancibia, Sandor
Andrés, Sergio
Morales Retamales, Paola
Herrera-Marschitz Muller, Mario
Herrera, Luisa
Lara Peñaloza, Hernán
Fiedler Temer, Jenny
Institución
Resumen
Background: Corticosterone reduction produced by adrenalectomy (ADX) induces apoptosis in dentate gyrus (DG) of the hippocampus, an effect related to an increase in the expression of the pro-apoptotic gene bax. However it has been reported that there is also an increase of the anti-apoptotic gene bcl-2, suggesting the promotion of a neuroprotective phenomenon, perhaps related to the expression of transforming growth factor β1 (TGF-β1). Thus, we have investigated whether TGF-β1 levels are induced by ADX, and whether apoptosis is increased by blocking the expression of TGF-β1 with an antisense oligonucleotide (ASO) administered intracerebrally in corticosterone depleted rats. Results: It was observed an increase of apoptosis in DG, 2 and 5 days after ADX, in agreement with a reduction of corticosterone levels. However, the effect of ADX on the number of apoptotic positive cells in DG was decreased 5 days after the lesion. In CA1-CA3 regions, the effect was only observed 2 days after ADX.