Artículos de revistas
The β-isoform of heat shock protein hsp-90 is structurally related with human microtubule-interacting protein Mip-90
Fecha
1999Registro en:
FEBS Letters, Volumen 457, Issue 3, 2018, Pages 343-347
00145793
10.1016/S0014-5793(99)01070-4
Autor
Cambiazo Ayala, Verónica
González, Mauricio
Isamit, Cristián
Maccioni Baraona, Ricardo
Institución
Resumen
Through major research advances in the study of cytoskeletal organization, an integrated view of the complexity of this system has emerged. Recent findings on the microtubule-interacting protein Mip-90, which associates with microtubules and actin filaments in different cell domains, have shed light on its roles in cytoskeletal regulation. In order to study structural features of Mip-90, we sequenced several peptide fragments. A comparative sequence analysis revealed a high degree of similarity between the primary structure of this protein and the human heat shock protein of 90 kDa (hsp-90). Taken together, the present studies indicate the identity between Mip-90 and the the β-isoform of hsp-90 (hsp-90β). Western blot assays with an anti-hsp-90 monoclonal antibody showed cross-reactivity of hsp-90 and Mip-90 affinity purified from HeLa cells. Furthermore, the observed structural identity of Mip-90 with the hsp-90β was sustained by immunoblot assays using monoclonal antibodies that spec
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