Artículos de revistas
Glucose and ethanol-dependent transcriptional regulation of the astaxanthin biosynthesis pathway in Xanthophyllomyces dendrorhous
Fecha
2011Registro en:
BMC Microbiology, Volumen 11,
14712180
14712180
10.1186/1471-2180-11-190
Autor
Marcoleta, Andrés
Niklitschek, Mauricio
Wozniak, Aniela
Lozano, Carla
Alcaíno Gorman, Jennifer
Baeza Cancino, Marcelo
Cifuentes Guzmán, Víctor
Institución
Resumen
Background: The yeast Xanthophyllomyces dendrorhous is one of the most promising and economically attractive natural sources of astaxanthin. The biosynthesis of this valuable carotenoid is a complex process for which the regulatory mechanisms remain mostly unknown. Several studies have shown a strong correlation between the carbon source present in the medium and the amount of pigments synthesized. Carotenoid production is especially low when high glucose concentrations are used in the medium, while a significant increase is observed with non-fermentable carbon sources. However, the molecular basis of this phenomenon has not been established. Results: In this work, we showed that glucose caused transcriptional repression of the three genes involved in the synthesis of astaxanthin from geranylgeranyl pyrophosphate in X. dendrorhous, which correlates with a complete inhibition of pigment synthesis. Strikingly, this regulatory response was completely altered in mutant strains that are inc