Artículos de revistas
Involvement of SchRabGDI1 from Solanum chilense in endocytic trafficking and tolerance to salt stress
Fecha
2017Registro en:
Plant Science 263 (2017) 1–11
Autor
San Martín Davison, Alex
Pérez Díaz, Ricardo
Soto, Flavia
Madrid Espinoza, José
González Villanueva, Enrique
Pizarro, Lorena
Norambuena Morales, Lorena
Tapia, Jaime
Tajima, Hiromi
Blumwaldd, Eduardo
Ruiz Lara, Simón
Institución
Resumen
Physiological responses of plants to salinity stress requires the coordinated activation of many genes. A saltinduced
gene was isolated from roots of the wild tomato species Solanum chilense and named SchRabGDI1 because
it encodes a protein with high identity to GDP dissociation inhibitors of plants. These proteins are regulators
of the RabGTPase cycle that play key roles in intracellular vesicular trafficking. The expression pattern of
SchRabGDI1 showed an early up–regulation in roots and leaves under salt stress. Functional activity of
SchRabGDI1 was shown by restoring the defective phenotype of the yeast sec19-1 mutant and the capacity of
SchRabGDI1 to interact with RabGTPase was demonstrated through BiFC assays. Expression of SchRabGDI1 in
Arabidopsis thaliana plants resulted in increased salt tolerance. Also, the root cells of transgenic plants showed
higher rate of endocytosis under normal growth conditions and higher accumulation of sodium in vacuoles and
small vesicular structures under salt stress than wild type. Our results suggest that in salt tolerant species such as
S. chilense, bulk endocytosis is one of the early mechanisms to avoid salt stress, which requires the concerted
expression of regulatory genes involved in vesicular trafficking of the endocytic pathway.