Artículo de revista
The effects of gold nanoparticles functionalized with beta-amyloid specific peptides on an in vitro model of blood–brain barrier
Fecha
2017Registro en:
Nanomedicine: NBM 13 (2017) 1645–1652
Autor
Ruffa, J.
Hüwelb, S.
Kogan Bocian, Marcelo
Simon, Ulrich
Joachim Gallab, Hans
Institución
Resumen
We studied the effect of gold nanoparticle (AuNP) size, surface charge, concentration and morphology on the integrity of the blood–brain
barrier (BBB) in a well-established in vitro model set-up. We focused on the effect of peptide functionalized hollow gold nanospheres and
gold nanorods, which selectively bind to amyloidogenic β-amyloid structures. These AuNP conjugates have already been successfully tested
as photothermal absorbers for potential application in Alzheimer's disease (AD) therapy in an in vitro set-up, but may exhibit a low passage
through the BBB due to their overall negative charge. Our results show that: (i) small (1.4 nm) AuNPs strongly affects the BBB integrity, (ii)
negative surface charge impedes BBB passage, and (iii) this charge effect caused by the peptide is compensated by covalent coupling to a
polyethylene glycol ligand stabilizing the particles in diluted manner.