Artículos de revistas
Spatial and temporal distribution of Patched-related protein in the Drosophila embryo
Fecha
2015Registro en:
Gene Expression Patterns 19 (2015) 120-128
DOI: 10.1016/j.gep.2015.10.002
Autor
Bolatto, Carmen
Parada, Cristina
Revello, Fiorella
Zúñiga, Alejandro
Cabrera, Pablo
Cambiazo Ayala, Verónica
Institución
Resumen
Patched-related (Ptr) encodes a protein with 12 potential transmembrane domains and a sterol-sensing domain that is closely related in predicted topology and domain organization to Patched, the canonical receptor of the Hedgehog pathway. Here we describe the production of an antibody specific for Drosophila Ptr and analyse its spatial and temporal distribution in the embryo. We find that at early developmental stages Ptr is predominantly localized at cell periphery but later on it becomes strongly and almost exclusively expressed in hemocytes. Interestingly Ptr null mutant embryos died without hatching. Our findings suggest that Ptr plays an essential function in Drosophila development, perhaps as a new receptor of embryonic hemocytes.