Artículos de revistas
Alloreactive Regulatory T Cells Allow the Generation of Mixed Chimerism and Transplant Tolerance
Fecha
2015Registro en:
Frontiers in Immunology | November 2015 | Volume 6 | Article 596
1664-3224
doi: 10.3389/fimmu.2015.00596
Autor
Ruiz, Paulina
Maldonado, Paula
Hidalgo, Yessia
Sauma Mahaluf, Daniela
Rosemblatt Silber, Mario César
Bono Merino, María Rosa
Institución
Resumen
The induction of donor-specific transplant tolerance is one of the main goals of modern immunology. Establishment of a mixed chimerism state in the transplant recipient has proven to be a suitable strategy for the induction of long-term allograft tolerance; however, current experimental recipient preconditioning protocols have many side effects, and are not feasible for use in future therapies. In order to improve the current mixed chimerism induction protocols, we developed a non-myeloablative bone-marrow transplant (NM-BMT) protocol using retinoic acid (RA)-induced alloantigen-specific Tregs, clinically available immunosuppressive drugs, and lower doses of irradiation. We demonstrate that RA-induced alloantigen-specific Tregs in addition to a NM-BMT protocol generates stable mixed chimerism and induces tolerance to allogeneic secondary skin allografts in mice. Therefore, the establishment of mixed chimerism through the use of donor-specific Tregs rather than non-specific immunosuppression could have a potential use in organ transplantation.