Artículos de revistas
Phospholipase C gamma and ERK1/2 Mitogen Activated Kinase Pathways are differentially modulated by Trypanosoma cruzi during tissue invasion in human placenta
Fecha
2013Registro en:
Experimental Parasitology 133 (2013) 12–17
doi 10.1016/j.exppara.2012.10.012
Autor
Castillo, Christian
Villarroel, Arturo
Duaso, Juan
Galanti Garrone, Norbel
Cabrera Vallejos, Gonzalo
Maya Arango, Juan
Kemmerling Weis, Ulrike
Institución
Resumen
Chagas‘ disease is caused by the haemophlagelated protozoan Trypanosoma cruzi (T. cruzi). During congenital
transmission the parasite breaks down the placental barrier, however studies about the physiopathology
of this process are scarce. Different signal transduction pathways are involved during cell
invasion of the parasite. However, the possible role of those processes during tissue infection has not
been studied. In the present study we analyzed the modulation of two signal transduction pathways,
PLC-c and ERK1/2 MAPK, during ex vivo infection of human placental chorionic villi explants.
Chorionic villi from healthy woman placentas were incubated in the presence or absence of 105 or 106
T. cruzi trypomastigotes (DM28c strain) with or without specific inhibitors for each pathway. Effective
infection was tested determining parasite DNA by PCR. The activation of PLC-c and ERK1/2 MAPK signaling
pathways was determined by western blotting and immunofluorescence.
The low concentration of T. cruzi trypomastigotes activates both signaling pathways; however, the high
concentration of parasite induces a modest activation of the PLC-c pathway and impairs the ERK1/2
MAPK pathway activation. Interestingly, inhibition of any of those signaling pathways did not prevent
parasite infection, as it was previously shown in cell cultures. We conclude that both signal transduction
pathways are modulated during ex vivo T. cruzi infection of human placental chorionic villi explants.