Artículos de revistas
The long polar fimbriae of STEC O157:H7 induce expression of pro-inflammatory markers by intestinal epithelial cells
Fecha
2013Registro en:
Veterinary Immunology and Immunopathology 152 (2013) 126– 131
DOI: 10.1016/j.vetimm.2012.09.017
Autor
Farfán, Mauricio J.
Cantero, Lidia
Vergara, Alejandra
Vidal Ávarez, Roberto
Torres, Alfredo G.
Institución
Resumen
Infection with Shiga toxin-producing Escherichia coli (STEC) O157:H7 is characterized by
acute inflammation of the colonic mucosa. STEC O157:H7 contains two non-identical loci
encoding long polar fimbriae (Lpf), which play a role in the STEC colonization of the intestinal
epithelial cells. However, no information is available regarding the involvement of Lpf in
the STEC-induced host inflammatory response. Hence, in this study we assess the role of Lpf
as an inducer of inflammation on intestinal epithelial cells. Secretion of pro-inflammatory
cytokines in response to STEC wild type and lpf isogenic mutants was evaluated on intestinal
T84 cells. Of the 27 cytokines assayed, IL-6, IL-8, IL-15, FGF, GM-CSF and IP-10 were significantly
reduced, when compared to the wild-type strain, in the lpfA1 lpfA2 double mutant.
Further, the host intracellular signaling pathways activated in response to Lpf were determined
by using an array containing genes representative of 18 different signal transduction
pathways. The analysis indicated that the NF- B pathway is activated in response to Lpfexpressing
STEC. Therefore, our study supports the role of Lpf as a STEC factor mediating
intestinal inflammation.