dc.creator | García, Víctor | |
dc.creator | Devoto, Luigi | |
dc.creator | Kohen Skop, Paulina | |
dc.creator | Maldonado, Carola | |
dc.creator | Sierralta, Walter | |
dc.creator | Muñoz Gallardo, Alex | |
dc.creator | Villarroel, Claudio | |
dc.creator | Strauss, Jerome F. | |
dc.date.accessioned | 2012-04-25T19:32:30Z | |
dc.date.accessioned | 2019-04-26T00:05:59Z | |
dc.date.available | 2012-04-25T19:32:30Z | |
dc.date.available | 2019-04-26T00:05:59Z | |
dc.date.created | 2012-04-25T19:32:30Z | |
dc.date.issued | 2012-03 | |
dc.identifier | FERTILITY AND STERILITY Volume: 97 Issue: 3 Pages: 707-U214 Published: MAR 2012 | |
dc.identifier | 0015-0282 | |
dc.identifier | DOI: 10.1016/j.fertnstert.2011.12.039 | |
dc.identifier | http://repositorio.uchile.cl/handle/2250/128950 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/2433271 | |
dc.description.abstract | Objective: To study in vivo the progesterone receptor (PR) expression levels in human granulosa cells (GCs) during the periovulatory period and the
affect of the protein kinase A (PKA) pathway on PR expression and cathepsin-L expression-activation.
Design: Experimental study.
Setting: University research unit.
Patient(s): Twenty-five women of reproductive age.
Intervention(s): Follicular fluid and GCs obtained from spontaneous cycles before and during the normal luteinizing hormone surge, and samples obtained
36 hours after human chorionic gonadotropin (hCG) administration in patients undergoing in vitro fertilization.
Main Outcome Measure(s): To determine PR, cathepsin-L messenger RNA (mRNA) analysis via real-time polymerase chain reaction, and protein of PR,
cathepsin-L, and PKA in human GCs.
Result(s): The Western blot analysis revealed that bands of PR (isoform A) were the most abundant and that mRNA (PR-A and PR-B) have a temporal
pattern of expression throughout the periovulatory period. The protein levels of PR and cathepsin-L were up-regulated by hCG. The abundance of PR was
diminished in the presence of PKA inhibitor, and cathepsin-L with PR receptor antagonist.
Conclusion(s): The transient expression of PR in human GCs of the preovulatory follicle suggests that PR and its ligand play a role in the activation of
cathepsin-L, which is presumably involved in the degradation of the follicular extracellular matrix during human ovulation. | |
dc.language | en | |
dc.publisher | Elsevier | |
dc.subject | Cathepsin -L | |
dc.title | Transient expression of progesterone receptor and cathepsin-l in human granulosa cells during the periovulatory period | |
dc.type | Artículos de revistas | |