Artículo de revista
Chromosomal size conservation through the cell cycle supports karyotype stability in Trypanosoma cruzi
Fecha
2007-05Registro en:
FEBS LETTERS, v.: 581, issue: 10, p.: 2022-2026, MAY 15 2007
0014-5793
Autor
Galindo Díaz, Mario
Sabaj Diez, Valeria
Espinoza, Ingrid
Toro, Gabriela Cecilia
Búa, Jaqueline
Grenet, Jose
López Solís, Remigio
Ruiz, Andres Mariano
Galanti Garrone, Norbel
Institución
Resumen
The Trypanosoma cruzi karyotype shows an extensive
chromosomal size polymorphism. Absence of condensed mitotic
chromosomes and chromatin fragility are characteristic
features of T. cruzi which would allow DNA breaks and chromosomal
rearrangements during cell proliferation. We have investigated
by pulsed field gel electrophoresis (PFGE) eventual
changes in chromosomal size during exponential and stationary
phases of T. cruzi epimastigotes in culture, in G0 trypomastigotes
and throughout the cell cycle in synchronized epimastigotes.
T. cruzi molecular karyotype was stable throughout the cell cycle
and during differentiation. Thus, the chromosomal size polymorphism
previously reported in T. cruzi contrasts with the stability
of the molecular karyotype observed here and suggests that chromosomal
rearrangements leading to changes in chromosomal
size are scarce events during the clonal propagation of this parasite