Artículo de revista
Changes in iron transporter divalent metal transporter 1 in proximal jejunum after gastric bypass
Fecha
2014Registro en:
World J Gastroenterol 2014 June 7; 20(21): 6534-6540
2219-2840
DOI: 10.3748/wjg.v20.i21.6534
Autor
Díaz Araya, Guillermo
Watkins Sepulveda, Juan Guillermo
Castro, Fabiola
Riffo, Andrea
Zúñiga, Roberta
Jans, Jaime
Villanueva, María E.
Marambio, Andrés
Institución
Resumen
AIM: To describe the variation that divalent metal
transporter 1 (DMT1) shows in patients after Rouxen-
Y gastric bypass (RYGB) surgery.
METHODS: Prospective and analytical study of DMT1
level at the brush border of proximal jejunum in patients
having undergone RYGB surgery. The mucosa of
proximal jejunum forming the gastrojejunal anastomosis
was biopsied during surgery and after 6 mo later
with an endoscopic biopsy. All the patients received
precise instructions regarding feeding and nutritional
supplementation. Both samples were processed at the
same time by immunohistochemistry and western blot.
Samples were analysed by a pathologist. For statistical
analysis, the χ 2 and Wilcoxon tests were used.
RESULTS: Sixteen patients were recruited, 13 of whom
completed the study. Twelve were women. Average age
and body mass index (BMI) were 44.1 and 40.4, respectively.
Both body weight and BMI decreased significantly
during the study period, with an average percent
excess weight loss (%EWL) of 60% ± 13.3% and an
average percent excess BMI loss (%EBMIL) of 79.6% ±
21.6%. Only two patients presented with mild anaemia
6 mo after surgery, but their ferritin levels stayed within
normal ranges. Staining for DMT1 showed a significant
increase in the cytoplasm of enterocytes located at the
tips of the villi (χ 2 = 6.03; p = 0.049). Nevertheless,
the total quantity of DMT1 decreased significantly (Z =
2.04; p = 0.04). Associated with these results, we observed
a significant increase in goblet cells in the villi 6
mo postoperatively (Z = -2.47; p = 0.013).
CONCLUSION: Six months after RYGB surgery, patients
exhibit an increase in DMT1 expression in the enterocytes
of the tips of the villi at the proximal jejunum.