Artículos de revistas
Supplementing female rats with DHA-lysophosphatidylcholine increases docosahexaenoic acid and acetylcholine contents in the brain and improves the memory and learning capabilities of the pups
Fecha
2010-03Registro en:
GRASAS Y ACEITES, 61 (1), ENERO-MARZO, 16-23, 2010
0017-3495
DOI: 10.3989/gya.053709
Autor
Valenzuela Bonomo, Carlos
Nieto, Susana
Sanhueza, Julio
Morgado, Nora
Rojas, Ingrid
Zañartu, Patricia
Institución
Resumen
DHA has been established. We propose that DHAcontaining
lysophosphatidylcholine (DHA-LPC), obtained
from DHA-rich eggs may be a suitable form of DHA and
choline (the precursor of acetylcholine) supplementation.
We evaluated the effectiveness of DHA-LPC to increase
DHA and acetylcholine concentration in the brain of pups
born from female rats supplemented with DHA-LPC before
and during pregnancy. We also evaluated the effect of DHA
supplementation on learning and memory capabilities of pups
through the Skinner test for operant conditioning. Female
Wistar rats received 40-day supplementation of DHA-LPC (8
mg DHA/kg b.w/daily.), before and during pregnancy. After
delivery, plasma, erythrocyte, liver, and adipose tissue DHA
and plasma choline were analyzed. Brains from 60 day-old
pups separated into frontal cortex, cerebellum, striatum,
hippocampus, and occipital cortex, were assessed for DHA,
acetylcholine, and acetylcholine transferase (CAT) activity.
Pups were subjected to the Skinner box test. DHA-LPC
supplementation produces higher choline and liver DHA
contents in the mother’s plasma and increases the pups’
DHA and acetylcholine in the cerebellum and hippocampus.
CAT was not modified by supplementation. The Skinner test
shows that pups born from DHA-LPC supplemented mothers
exhibit better scores of learning and memory than the
controls. Conclusion: DHA-LPC may be an adequate form for
DHA supplementation during the perinatal period.