Artículo de revista
Expression of proinflammatory cytokines in osteoarthritis of the temporomandibular joint
Fecha
2008-10Registro en:
ARCHIVES OF ORAL BIOLOGY, Volume: 53, Issue: 10, Pages: 910-915, 2008
0003-9969
Autor
Vernal Astudillo, Rolando
Velásquez, Edgar
Gamonal Aravena, Jorge Antonio
García Sanz, José A.
Silva, Augusto
Sanz, Mariano
Institución
Resumen
Objective: This study reports the expression of proinflammatory cytokines in temporomandibular
joint (TMJ) of patients affected with temporomandibular osteoarthritis (OA).
Design: In twelve OA of the TMJ (OA–TMJ) affected patients and in six healthy volunteer
subjects studied as control, the expression of IL1b (interleukin-1b), IL2, IL4, IL5, IL6, IL10,
IL12p35, IL12p40, IL17, IFNg (interferon-gamma), TNFa (tumor necrosis factor-alpha), and
TNFb mRNAs was evaluated. Using quantitative real-time RT-PCR technique, the cytokine
levels, reported as Ct (cycle threshold), DCt (Ct cytokine Ct 18S rRNA) and RQ (relative
quantification), in patient and control groups were compared.
Results: Expression of IL1b, IL2, IL12p35, IL12p40, IL17, TNFa, TNFb, and IFNg mRNAs was
significantly higher in patients as compared with controls. In particular, IL12 was the
predominant cytokine expressed in patients (IL12p35 RQ = 30.2 and IL12p40 RQ = 29.0).
Conversely, IL10 mRNA levels were higher in controls (RQ = 1.8).
Conclusions: These data suggest that not only IL1b, IFNg, and TNFa but also IL10, IL12, and
IL17 are involved in the OA–TMJ pathogenesis. Furthermore, an inflammatory response
characterised by the predominant expression of IL12 mRNA and down-regulated expression
of IL10 mRNA is associated with the degenerative changes observed in OA–TMJ.