Artículo de revista
Monounsaturated Fatty Acids Are Substrates for Aldehyde Generation in Tellurite-Exposed Escherichia coli
Fecha
2013Registro en:
BioMed Research International Volume 2013, Article ID 563756, 7 pages
Autor
Pradenas, Gonzalo A.
Díaz Vásquez, Waldo A.
Pérez Donoso, José
Vásquez, Claudio C.
Institución
Resumen
Reactive oxygen species (ROS) damage macromolecules and cellular components in nearly all kinds of cells and often generate
toxic intracellular byproducts. In this work, aldehyde generation derived from the Escherichia coli membrane oxidation as well as
membrane fatty acid profiles, protein oxidation, and bacterial resistance to oxidative stress elicitors was evaluated. Studies included
wild-type cells as well as cells exhibiting a modulated monounsaturated fatty acid (MUFA) ratio.The hydroxyaldehyde 4-hydroxy
2-nonenal was found to be most likely produced by E. coli, whose levels are dependent upon exposure to oxidative stress elicitors.
Aldehyde amounts and markers of oxidative damage decreased upon exposure to E. coli containing low MUFA ratios, which was
paralleled by a concomitant increase in resistance to ROS-generating compounds. MUFAs ratio, lipid peroxidation, and aldehyde
generation were found to be directly related; that is, the lower the MUFAs ratio, the lower the peroxide and aldehyde generation
levels.These results provide additional evidence about MUFAs being targets for membrane lipid oxidation and their relevance in
aldehyde generation.