Artículos de revistas
Transforming Growth Factor-beta and All-Trans Retinoic Acid Generate Ex Vivo Transgenic Regulatory T Cells With Intestinal Homing Receptors
Fecha
2009-07Registro en:
TRANSPLANTATION PROCEEDINGS, Volume: 41, Issue: 6, Pages: 2670-2672, 2009
0041-1345
Autor
Moore, C.
Sauma Mahaluf, Daniela
Morales Peña, José
Bono Merino, María Rosa
Rosemblatt Silber, Mario César
Fierro, J. A.
Institución
Resumen
CD4 CD25 Foxp3 regulatory T cells (Treg) mediate immunologic self-tolerance and
suppress immune responses. In the gut, a subset of dendritic cells is specialized to induce
Treg in a transforming growth factor- (TGF- )- and retinoic acid (RA)-dependent
manner. The aim of this study was to establish if RA synergizing with TGF- induced
antigen specific CD4 CD25high Foxp3 Treg portraying gut homing receptors. Splenic
CD4 CD25 Foxp3 naïve T cells from DO11.10 mice were cocultured with splenic
CD11c dendritic cells from Balb/c mice in the presence of TGF- , RA, and low levels of
an antigenic peptide. After 5 days of culture, cells were analyzed for the expression of
Foxp3 and the gut homing receptors CCR9 and 4 7.
The number of Foxp3 T cells generated with TGF- and RA was at least 3 times higher
than in the cultures with TGF- alone and 15 times higher than in controls without
exogenous cytokines. Also, supplementation of the cultures with RA induced the
expression of the intestinal homing receptors CCR9 and 4 7. Our results showed that
coculture of naïve T cells with antigen-presenting cells in the presence of TGF- and RA
represents a powerful approach to generate Treg with specific homing receptors.