Artículo de revista
Expression profiling identifies novel Hh/Gli-regulated genes in developing zebrafish embryos
Fecha
2007-12-11Registro en:
GENOMICS, Volume: 91, Issue: 2, Pages: 165-177, 2008
0888-7543
Autor
Bergeron, Sadie A.
Milla, Luis A.
Villegas, Rosario
Shen, Meng-Chieh
Burgess, Shawn M.
Allende Connelly, Miguel
Karlstrom, Rolf O.
Palma Alvarado, Verónica
Institución
Resumen
The Hedgehog (Hh) signaling pathway plays critical instructional roles during embryonic development. Misregulation of Hh/Gli signaling is a
major causative factor in human congenital disorders and in a variety of cancers. The zebrafish is a powerful genetic model for the study of Hh
signaling during embryogenesis, as a large number of mutants that affect different components of the Hh/Gli signaling system have been
identified. By performing global profiling of gene expression in different Hh/Gli gain- and loss-of-function scenarios we identified known
(e.g., ptc1 and nkx2.2a) and novel Hh-regulated genes that are differentially expressed in embryos with altered Hh/Gli signaling function. By
uncovering changes in tissue-specific gene expression, we revealed new embryological processes that are influenced by Hh signaling. We thus
provide a comprehensive survey of Hh/Gli-regulated genes during embryogenesis and we identify new Hh-regulated genes that may be targets of
misregulation during tumorigenesis.