bachelorThesis
ADN libre plasmático como marcador molecular en lesiones pre-neoplásicas de cuello uterino y su asociación con VPH
Fecha
2012Registro en:
TEME 0045 2012
Autor
González Melo, Mary Julieth
Institución
Resumen
Introduction: The concentration of cell free DNA in plasma has been investigated as a tumor biomarker in several cancers. However, few studies evaluating the concentration cell free DNA in cervical cancer patients and to date no studies in patients with pre-cancerous cervical lesions.
Objective: Establish the association between the concentration of cell free DNA and the degree of cervical neoplasia and evaluate their possible association with viral type.
Methodology: This is an Analytical prevalence study with a non-probability sampling. The cell free DNA was quantified in plasma by real-time PCR of 92 patients who had some cervical intraepithelial lesion, confirmed by biopsy in different institutions of Bogotá. In addition to this was performed genotyping of virus by Reverse LineBlot .
Results: The concentration of Cell free DNA in patients with pre-cancerous lesions was 4515 ± 16402 ng / µl (mean ± SD), LSIL was 5188.7 ± 14876.5 ng/ µl (mean ± SD), in patients with HSIL was 830.3 ± 1515,508 ng / L (mean ± SD), in patients with negative results was 7024.7 ± 24107.5 ng / L (mean ± SD). The serotypes found in the study population showed no association with the Cell free DNA concentration.
DISCUSSION: The results showed that the absolute concentration of Cell free DNA in plasma has no predictive value to differentiate the types of pre-neoplastic lesions of the cervix, since no significant differences in the concentration of free DNA in plasma of different progressive stages of cervical cancer (p, 0.57, gl, 3 alpha 0.05) in the same way serotype does not contribute to explain the concentration of free DNA.