| dc.description | RESUMEN: la esponja marina Aplysina fistularis fue colectada en Isla Larga, bahía de Mochima, Venezuela, cortada en
trozos y sumergida en metanol, filtrada al vacío y extraída cada 24 h. El extracto acuoso fue particionado con acetato de etilo
(FAE). FAE fue separado por cromatografía sobre sílica gel obteniéndose 15 fracciones; a partir de la F5 se adquirieron 15
fracciones y de la F5-5 se aisló el terpeno 3,5-dibromo-1-hidroxi-4,4-dimetoxi- 2,5-ciclohexadien-1-acetamida elucidada por
RMN 13C y 1H con actividad antibacteriana. Las pruebas químicas revelaron la presencia de alcaloides y esteroles en las
fracciones probadas.
ABSTRACT: A specimen of the marine sponge Aplysina fistularis collected off the coast of Isla Larga, in the Bay of
Mochima, Venezuela (10°20’-10°24’N, 64°19’-64°22’W) was cut into pieces and submerged in methanol for 24 hours, thus
resulting an exudate suspension that was vacuum-filtered and separated into fractions with ethyl acetate. The fractions in
ethyl acetate (FEA) were in turn separated by silica gel chromatography to produce 15 fractions, from which further fractions
were obtained. The subfraction resulting from F5, videlicet, F5-5, yielded a precipitate crystal identified by 13C- and 1H-NMR as
the terpen 3,5 dibromo-1-hydroxy-4,4-dimethoxy-2,5-cyclohexadien-1-acetamide, whose antibacterial effect was positively
tested against several bacterium strains. Chemical assays also revealed the presence of alkaloids and sterols in the fractions
assayed. | |
