Artículos de revistas
Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
Autor
GREGORIO DEL CARMEN GODOY HERNANDEZ
ELIDE AVILES BERZUNZA
MILDRED RUBI CARRILLO PECH
FELIPE AUGUSTO VAZQUEZ FLOTA
Institución
Resumen
Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation.
Materias
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