"The purpose of this work is to analyze the optical, chemical and biological behavior of four chemicals similar to 2,2’-thiodiethanol (TDE), in the perspective of their use as mounting media for high-resolution fluorescence microscopy. TDE, the model molecule in this work, was shown to possess many properties that make it an excellent fluid for the preparation of microscopy slides. Its drawback is the lack of compatibility with the most popular reagent for acting staining, the toxin phalloidin. Due to the relevance of phalloidin in terms of quality of actin decoration, simplicity of protocol and popularity, our aim is to find a compound similar to TDE, in terms of optical properties and structure that would allow the phalloidin - conjugated fluorosphes to be used. Three molecules with structural affinity to TDE were proposed as mounting media for fluorescent microscopy: Butyl sulfone (BTS), 3,3'-thiodipropanol (TDP), 2,2'-sulfonyldiethanol (SDE). In this work, the molecules were tested for solubility in water and their refractive indices were measured at different concentrations in water, finding a linear relation between the refractive index and the concentration. Absorption and fluorescence spectra of four different fluorophores in PBS, and in the analyzed mounting media –TDE and TDP– have been obtained. Cells stained with conjugated phalloidin were mounted using the previously mentioned media and imaged in a confocal fluorescence microscope. Fluorescence of conjugated phalloidin was not observed when cells were mounted in SDE. Labeled cells mounted in TDP showed fluorescence labeling and preserved their f-actin structures.”