Artículos de revistas
In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach
Fecha
2016-05Registro en:
Mateo Fernández, Marcos; Merinas Amo, Tania; Moreno Millán, Miguel; Alonso Moraga, Ángeles; Demyda-peyrás, Sebastian; In Vivo and In Vitro Genotoxic and Epigenetic Effects of Two Types of Cola Beverages and Caffeine: A Multiassay Approach; Hindawi Publishing Corporation; BioMed Research International; 2016; 5-2016; 1-15
2314-6133
2314-6141
CONICET Digital
CONICET
Autor
Mateo Fernández, Marcos
Merinas Amo, Tania
Moreno Millán, Miguel
Alonso Moraga, Ángeles
Demyda-peyrás, Sebastian
Resumen
The aim of this work was to assess the biological and food safety of two different beverages: Classic Coca Cola (CCC) and CaffeineFree Coca Cola (CFCC). To this end, we determined the genotoxicological and biological effects of different doses of lyophilised CCC and CFCC and Caffeine (CAF), the main distinctive constituent. Their toxic/antitoxic, genotoxic/antigenotoxic, and chronic toxicity (lifespan assay) effects were determined in vivo using the Drosophila model. Their cytotoxic activities were determined using the HL-60 in vitro cancer model. In addition, clastogenic DNA toxicity was measured using internucleosomal fragmentation and SCGE assays. Their epigenetic effects were assessed on the HL-60 methylation status using some repetitive elements. The experimental results showed a slight chemopreventive effect of the two cola beverages against HL-60 leukaemia cells, probably mediated by nonapoptotic mechanisms. Finally, CCC and CAF induced a global genome hypomethylation evaluated in LINE-1 and Alu M1 repetitive elements. Overall, we demonstrated for the first time the safety of this famous beverage in in vivo and in vitro models.