Artículos de revistas
Nitrated fatty acids: synthesis and measurement
Woodcock, Steven R.; Bonacci, Gustavo Roberto; Gelhaus, Stacy L.; Schopfer, Francisco J.; Nitrated fatty acids: synthesis and measurement ; Elsevier Inc; Free Radical Biology and Medicine; 59; 11-2012; 14-26
Woodcock, Steven R.
Bonacci, Gustavo Roberto
Gelhaus, Stacy L.
Schopfer, Francisco J.
Nitrated fatty acids are the product of nitrogen dioxide reaction with unsaturated fatty acids. The discovery of peroxynitrite and peroxidase-induced nitration of biomolecules led to the initial reports of endogenous nitrated fatty acids. These species increase during ischemia/reperfusion, but concentrations are often at or near the limits of detection. Here, we describe multiple methods for nitrated fatty acid synthesis and sample extraction from complex biological matrices and a rigorous method of qualitative and quantitative detection of nitrated fatty acids by liquid chromatography–mass spectrometry. In addition, optimized instrument conditions and caveats regarding data interpretation are discussed.