info:eu-repo/semantics/article
Macrophages and galectin 3 play critical roles in CVB3-induced murine acute myocarditis and chronic fibrosis
Fecha
2015-08Registro en:
Jaquenod de Giusti, Carolina; Ure, Agustin Enrique; Rivadeneyra, Leonardo; Schattner, Mirta Ana; Gomez, Ricardo Martin; Macrophages and galectin 3 play critical roles in CVB3-induced murine acute myocarditis and chronic fibrosis; Academic Press Ltd - Elsevier Science Ltd; Journal of Molecular and Cellular Cardiology; 85; 8-2015; 58-70
0022-2828
CONICET Digital
CONICET
Autor
Jaquenod de Giusti, Carolina
Ure, Agustin Enrique
Rivadeneyra, Leonardo
Schattner, Mirta Ana
Gomez, Ricardo Martin
Resumen
Macrophage influx and galectin 3 production have been suggested as major players driving acute inflammation and chronic fibrosis in many diseases. However, their involvement in the pathogenesis of viral myocarditis and subsequent cardiomyopathy are unknown. Our aim was to characterise the role of macrophages and galectin 3 on survival, clinical course, viral burden, acute pathology, and chronic fibrosis in coxsackievirus B3 (CVB3)-induced myocarditis. Our results showed that C3H/HeJ mice infected with CVB3 and depleted of macrophages by liposome-encapsulated clodronate treatment compared with infected untreated mice presented higher viral titres but reduced acute myocarditis and chronic fibrosis, compared with untreated infected mice. Increased galectin 3 transcriptional and translational expression levels correlated with CVB3 infection in macrophages and in non-depleted mice. Disruption of the galectin 3 gene did not affect viral titres but reduced acute myocarditis and chronic fibrosis compared with C57BL/6J wild-type mice. Similar results were observed after pharmacological inhibition of galectin 3 with N-acetyl-. d-lactosamine in C3H/HeJ mice. Our results showed a critical role of macrophages and their galectin 3 in controlling acute viral-induced cardiac injury and the subsequent fibrosis. Moreover, the fact that pharmacological inhibition of galectin 3 induced similar results to macrophage depletion regarding the degree of acute cardiac inflammation and chronic fibrosis opens up the possibility of new pharmacological strategies for viral myocarditis.