Artículos de revistas
Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
Fecha
2017-09Registro en:
Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Gammella, Mariela; Digiacomo, Sebastian; et al.; Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle; Public Library of Science; Plos One; 12; 9; 9-2017; 1-16; e0185184
1932-6203
CONICET Digital
CONICET
Autor
Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia Ana
Gammella, Mariela
Digiacomo, Sebastian
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia Ines
Resumen
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.