Artículos de revistas
Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens
Date
2014-02Registration in:
Mazzolini Rizzo, Guillermo Daniel; Alaniz, Laura Daniela; Rizzo, Manglio Miguel; Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens; Springer; Methods in Molecular Biology (clifton, N.j.); 2-2014; 41-44
1064-3745
CONICET Digital
CONICET
Author
Rizzo, Manglio Miguel
Alaniz, Laura Daniela
Mazzolini Rizzo, Guillermo Daniel
Abstract
Ex vivo-generated antigen-loaded dendritic cells (DC) have been shown to be immunogenic in patients with cancer. Loading DC with autologous whole tumor antigens is a strategy to arm DC against tumor without human leukocyte antigen (HLA) restriction. Furthermore, this approach allows the presentation of a full antigen range to the immune system. We describe the methods to obtain whole antigens from autologous tumor tissues in order to load DC generated ex vivo from patients with gastrointestinal cancer.