Artículos de revistas
A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
Fecha
2017-02Registro en:
Camara, María de los Milagros; Bouvier, León Alberto; Reigada, Chantal; Di Girolamo, Fabio Augusto; Martínez Sayé, Melisa Soledad; et al.; A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi; Czech Academy of Sciences. Institute of Parasitology; Folia Parasitologica; 64; 2-2017; 1-6
0015-5683
1803-6465
CONICET Digital
CONICET
Autor
Camara, María de los Milagros
Bouvier, León Alberto
Reigada, Chantal
Di Girolamo, Fabio Augusto
Martínez Sayé, Melisa Soledad
Pereira, Claudio Alejandro
Resumen
Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases.