info:eu-repo/semantics/article
Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8
Fecha
2014-03Registro en:
Soria, Leandro Raul; Marrone, Julieta; Molinas, Sara Maria; Lehmann Mantaras, Guillermo Luis; Calamita, Giuseppe; et al.; Lipopolysaccharide impairs hepatocyte ureagenesis from ammonia: Involvement of mitochondrial aquaporin-8; Elsevier; FEBS Letters; 588; 9; 3-2014; 1686-1691
0014-5793
Autor
Soria, Leandro Raul
Marrone, Julieta
Molinas, Sara Maria
Lehmann Mantaras, Guillermo Luis
Calamita, Giuseppe
Marinelli, Raul Alberto
Resumen
We recently reported that hepatocyte mitochondrial aquaporin-8 (mtAQP8) channels facilitate the uptake of ammonia and its metabolism into urea. Here we studied the effect of bacterial lipopolysaccharides (LPS) on ammonia-derived ureagenesis. In LPS-treated rats, hepatic mtAQP8 protein expression and diffusional ammonia permeability (measured utilizing ammonia analogues) of liver inner mitochondrial membranes were downregulated. NMR studies using 15N-labeled ammonia indicated that basal and glucagon-induced ureagenesis from ammonia were significantly reduced in hepatocytes from LPS-treated rats. Our data suggest that hepatocyte mtAQP8-mediated ammonia removal via ureagenesis is impaired by LPS, a mechanism potentially relevant to the molecular pathogenesis of defective hepatic ammonia detoxification in sepsis.