Artículos de revistas
Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study
Fecha
2000-02Registro en:
Pincelli, Michelina Marta; Levstein, Patricia Rebeca; Fidelio, Gerardo Daniel; Gennaro, Ana Maria; Cholesterol-induced alterations of the packing properties of gangliosides: an EPR study; Elsevier Ireland; Chemistry And Physics Of Lipids; 104; 2; 2-2000; 193-206
0009-3084
CONICET Digital
CONICET
Autor
Pincelli, Michelina Marta
Levstein, Patricia Rebeca
Fidelio, Gerardo Daniel
Gennaro, Ana Maria
Resumen
The effect of cholesterol (Chol) on two kinds of glycolipid assemblies, one composed of monosialogangliosides (G(M1a)) and the other formed by a natural mixture of bovine brain gangliosides (TBG), has been analysed. The experimental approach involves spin label electron paramagnetic resonance (EPR) in aqueous lipid dispersions. The employment of a hydrosoluble spin label and a `quencher´ of the EPR signal that is not able to permeate lipid interfaces, allowed us to conclude that G(M1a)/Chol mixtures give rise to vesicles at Chol proportions for which TBG/Chol mixtures form micelles. The use of different liposoluble spin labels reveals that cholesterol produces a straightening of the hydrocarbon chains in both lipid systems. In GM1a/Chol mixtures, this feature is more pronounced and it is coupled with a decrease in polarity at the chain ends.