info:eu-repo/semantics/article
Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy
Fecha
2013Registro en:
Heusschen, Roy; Freitag, Nancy; Tirado González, Irene; Barrientos, Gabriela Laura; Moschansky, Petra; et al.; Profiling Lgals9 splice variant expression at the fetal-maternal interface: implications in normal and pathological human pregnancy; Oxford University Press; Biology Of Reproduction; 88; 1; 2013; 1-10; 22
0006-3363
1529-7268
Autor
Heusschen, Roy
Freitag, Nancy
Tirado González, Irene
Barrientos, Gabriela Laura
Moschansky, Petra
Muñoz Fernández, Raquel
Leno Durán, Ester
Klapp, Burghard F.
Thijssen, Victor L.J.L.
Blois, Sandra M.
Resumen
Disruption of fetal-maternal tolerance mechanisms can contribute to pregnancy complications, including spontaneous abortion. Galectin-9 (LGALS9), a tandem repeat lectin associated with immune modulation, is expressed in the endometrium during the mid and late secretory phases and in decidua during human early pregnancy. However, the role of LGALS9 during pregnancy remains poorly understood. We used real-time PCR and immunohistochemical staining to analyze the expression of Lgals9/LGALS9 during mouse gestation as well as in human tissues obtained from normal pregnancy and spontaneous abortions. In mice, three Lgals9 splice variants were detected, the expression of which was differentially regulated during gestation. Furthermore, decidual Lgals9 expression was deregulated in a mouse model of spontaneous abortion, whereas placental levels did not change. We further found that the LGALS9 D5 isoform suppresses interferon gamma production by decidual natural killer cells. In human patients, six Lgals9 splice variants were detected, and a decrease in Lgals9 D5/10 was associated with spontaneous abortion. Altogether, these results show a differential regulation of Lgals9 isoform expression during normal and pathological pregnancies and designate Lgals9 as a potential marker for adverse pregnancy outcomes.