info:eu-repo/semantics/article
Alkaloid profiling and anticholinesterase activity of South American Lycopodiaceae species
Fecha
2013-01Registro en:
Konrath, Eduardo Luis; Ortega, María Gabriela; de Loreto Bordignon, Sergio ; Anders Apel, Miriam; Henriques, Amelia Teresinha; et al.; Alkaloid profiling and anticholinesterase activity of South American Lycopodiaceae species; Taylor & Francis Ltd; Journal Of Enzyme Inhibition And Medicinal Chemistry; 28; 1; 1-2013; 93-97
1475-6366
1475-6374
Autor
Konrath, Eduardo Luis
Ortega, María Gabriela
de Loreto Bordignon, Sergio
Anders Apel, Miriam
Henriques, Amelia Teresinha
Cabrera, Jose Luis
Resumen
The alkaloid extracts of four Huperzia and one Lycopodiella species, from Brazilian habitats, were tested for their in vitro anticholinesterase activities. IC50 values showed a potent acetylcholinesterase inhibition for H. reflexa (0.11 ± 0.05 μg/mL), followed by H. quadrifariata (2.0 ± 0.3 μg/mL), H. acerosa (5.5 ± 0.9 μg/mL), H. heterocarpon (25.6 ± 2.7 μg/mL) and L. cernua (42.6 ± 1.5 μg/mL). A lower inhibition of butyrylcholinesterase was observed for all species with the exception of H. heterocarpon (8.3 ± 0.9 μg/mL), whose alkaloid extract presented a selectivity for pseudocholinesterase. Moreover, the chemical study of the bioactive extracts performed by GC-MS, revealed the presence of a number of Lycopodium alkaloids belonging to the lycopodane, flabellidane and cernuane groups. Surprisingly, the potent acetylcholinesterase inhibitors huperzines A and B were not detected in the extracts, suggesting that other alkaloids may be responsible for such an effect.