Artículos de revistas
High passage numbers induce resistance to apoptosis in C2C12 muscle cells
Fecha
2013-01Registro en:
Pronsato, Lucía; la Colla, Anabela Belén; Ronda, Ana Carolina; Milanesi, Lorena Magdalena; Boland, Ricardo Leopoldo; et al.; High passage numbers induce resistance to apoptosis in C2C12 muscle cells; Sociedad Latinoamericana de Microscopía Electrónica; Biocell; 37; 1; 1-2013; 1-9
0327-9545
Autor
Pronsato, Lucía
la Colla, Anabela Belén
Ronda, Ana Carolina
Milanesi, Lorena Magdalena
Boland, Ricardo Leopoldo
Vasconsuelo, Andrea Anahi
Resumen
Cell lines with high passage numbers exhibit alterations in cell Morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.