info:eu-repo/semantics/article
Molecular recognition of an acyl-enzyme intermediate on the lipase B from Candida antarctica
Fecha
2017-03-28Registro en:
Toledo, Victoria; Llerena Suster, Carlos Rafael; Ferreira, María Luján; Collins, Sebastián Enrique; Briand, Laura Estefania; Molecular recognition of an acyl-enzyme intermediate on the lipase B from Candida antarctica; Royal Society of Chemistry; Catalysis Science and technology; 7; 9; 28-3-2017; 1953-1964
2044-4761
CONICET Digital
CONICET
Autor
Toledo, Victoria
Llerena Suster, Carlos Rafael
Ferreira, María Luján
Collins, Sebastián Enrique
Briand, Laura Estefania
Resumen
This investigation provides evidences of the acyl enzyme species involved in theinteraction of R/S-ketoprofen with the lipase B from Candida antarctica. The interaction between the profen and the enzyme was studied by in situ Time-Resolved ATR-FTIR both in static and transient conditions. Particularly, modulation excitation spectroscopy (MES) with phase sensitive detection (PSD) allowed to univocally distinguishing the signals belonging to the interaction between ketoprofen and the enzyme from the strong background signals. These experimental tools coupled with theoretical DFT analysis allowed to propose various species due to the interaction of ketoprofen with serine through H bonding (without reaction) and the acyl enzyme species (ester bond formation) that are the intermediates in the biocatalytic assisted esterification andhydrolysis using lipases.