Artículos de revistas
Effect of a Pseudomonas fluorescens tailocin against phytopathogenic Xanthomonas observed by atomic force microscopy
Fecha
2017-08Registro en:
Fernandez, Maricruz; Godino, Agustina; Principe, Analia; Morales, Gustavo Marcelo; Fischer, Sonia Elizabeth; Effect of a Pseudomonas fluorescens tailocin against phytopathogenic Xanthomonas observed by atomic force microscopy; Elsevier Science; Journal of Biotechnology; 256; 8-2017; 13-20
0168-1656
CONICET Digital
CONICET
Autor
Fernandez, Maricruz
Godino, Agustina
Principe, Analia
Morales, Gustavo Marcelo
Fischer, Sonia Elizabeth
Resumen
Phage tail-like bacteriocins, called tailocins, represent a class of protein complexes produced by a multitude of bacteria. Pseudomonas fluorescens SF4c, a strain isolated from wheat rhizosphere, produces a bacteriocin similar to phage tail-like pyocins of Pseudomonas aeruginosa. This tailocin has antimicrobial activity against several phytopathogenic strains of the genus Xanthomonas and Pseudomonas. In this work, the effect of the SF4c tailocin on the phytopathogenic strain X. axonopodis pv vesicatoria Xcv Bv5–4a was analyzed through Atomic Force Microscopy (AFM). We demonstrated that tailocins adhere and cause damage to the cell envelope of strain Xcv Bv5-4a. This results in a rapid leakage of intracellular materials, with the subsequent decrease of cell volume. Finally, lysis of sensitive bacteria occurs. This study provides, to our knowledge, the first evidence about the effect of a tailocin analyzed by AFM. Further studies are in progress to evaluate the use of SF4c tailocin in the biocontrol of bacterial spot on tomato.