Artículos de revistas
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
Fecha
2013-12Registro en:
Lucero Estrada, Cecilia Stella Marys; Alcaráz, Lucia E.; Satorres, Sara E.; Manfredi, Eduardo; Velázquez, Lidia del Carmen; Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 4; 12-2013; 1155-1161
1517-8382
1678-4405
CONICET Digital
CONICET
Autor
Lucero Estrada, Cecilia Stella Marys
Alcaráz, Lucia E.
Satorres, Sara E.
Manfredi, Eduardo
Velázquez, Lidia del Carmen
Resumen
An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.