info:eu-repo/semantics/article
PPARγ activation regulates lipid droplet formation and lactate production in rat Sertoli cells
Fecha
2017-04Registro en:
Gorga, Agostina; Rindone, Gustavo Marcelo; Regueira, Mariana; Pellizzari, Eliana Herminia; Camberos, Maria del Carmen; et al.; PPARγ activation regulates lipid droplet formation and lactate production in rat Sertoli cells; Springer; Cell and Tissue Research; 369; 3; 4-2017; 611-624
0302-766X
CONICET Digital
CONICET
Autor
Gorga, Agostina
Rindone, Gustavo Marcelo
Regueira, Mariana
Pellizzari, Eliana Herminia
Camberos, Maria del Carmen
Cigorraga, Selva Beatriz
Riera, Maria Fernanda
Galardo, Maria Noel Lujan
Meroni, Silvina Beatriz
Resumen
Sertoli cells provide the structural and nutritional support for germ cell development; they actively metabolize glucose and convert it to lactate, which is an important source of energy for germ cells. Furthermore, Sertoli cells can oxidize fatty acids, a metabolic process that is assumed to fulfill their own energy requirements. Fatty acids are stored as triacylglycerides within lipid droplets. The regulation of fatty acid storage in conjunction with the regulation of lactate production may thus be relevant to seminiferous tubule physiology. Our aim is to evaluate a possible means of regulation by the PPARγ activation of lipid droplet formation and lactate production. Sertoli cell cultures obtained from 20-day-old rats were incubated with Rosiglitazone (10 μM), a PPARγ activator, for various periods of time (6, 12, 24 and 48 h). Increased triacylglycerides levels and lipid droplet content were observed, accompanied by a rise in the expression of genes for proteins involved in fatty acid storage, such as the fatty acid transporter Cd36, glycerol-3-phosphate-acyltransferases 1 and 3, diacylglycerol acyltransferase 1 and perilipins 1, 2 and 3, all proteins that participate in lipid droplet formation and stabilization. However, PPARγ activation increased lactate production, accompanied by an augmentation in glucose uptake and Glut2 expression. These results taken together suggest that PPARγ activation in Sertoli cells participates in the regulation of lipid storage and lactate production thereby ensuring simultaneously the energetic metabolism for the Sertoli and germ cells.