Artículos de revistas
In vitro plantlets regeneration of Schinopsis balansae (Anacardiaceae)
Fecha
2003-12Registro en:
Sansberro, Pedro Alfonso; Rey de Badaró, Hebe Yolanda; Mroginski, Luis Amado; Luna, Claudia Verónica; In vitro plantlets regeneration of Schinopsis balansae (Anacardiaceae); Springer Berlin Heidelberg; Trees; 17; 6; 12-2003; 542-546
0931-1890
1432-2285
CONICET Digital
CONICET
Autor
Sansberro, Pedro Alfonso
Rey de Badaró, Hebe Yolanda
Mroginski, Luis Amado
Luna, Claudia Verónica
Resumen
A protocol for the in vitro regeneration of rooted plants from nodal single bud segments of 10-year-old Schinopsis balansae trees was developed. Nodal segments were harvested from actively growing shoots of plants grown from seeds and maintained in pots under greenhouse conditions, and from epicormic shoots obtained by forced flushing of branches. Culture of nodal segments on nutrient medium containing the mineral salts and vitamins of Murashige and Skoog medium at 1/4 strength (1/4 MS), supplemented with 100 mg l−1 ascorbic acid, 3% sucrose, and 5–15 μM 6-benzyladenine resulted in regeneration of multiple shoots. Rooting of regenerated shoots was observed in 1/4 MS medium with vermiculite as the substrate and supplemented with 7.5 μM indolbutyric acid.