Artículos de revistas
Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays
Fecha
2017-04Registro en:
Soloneski, Sonia Maria Elsa; Ruiz de Arcaute, Celeste; Nikoloff, Noelia; Larramendy, Marcelo Luis; Genotoxicity of the herbicide imazethapyr in mammalian cells by oxidative DNA damage evaluation using the Endo III and FPG alkaline comet assays; Springer Heidelberg; Environmental Science and Pollution Research; 24; 11; 4-2017; 10292-10300
0944-1344
1614-7499
CONICET Digital
CONICET
Autor
Soloneski, Sonia Maria Elsa
Ruiz de Arcaute, Celeste
Nikoloff, Noelia
Larramendy, Marcelo Luis
Resumen
We evaluated the role of oxidative stress in the genotoxic damage induced by imazethapyr (IMZT) and its formulation Pivot® in mammalian CHO-K1 cell line. Using the alkaline comet assay, we observed that a concentration of 0.1 μg/mL of IMZT or Pivot® was able to induce DNA damage by increasing the frequency of damaged nucleoids. To test whether the DNA lesions were caused by oxidative stress, the DNA repair enzymes endonuclease III (Endo III) and formamidopyrimidine-DNA glycosylase (Fpg), which convert base damage to strand breaks, were used. Our results demonstrate that after treatment of CHO-K1 cells with the pure active ingredient as well as the commercial formulation Pivot®, an increase in DNA strand breaks was observed after incubation of both Endo III and Fpg enzymes, indicating that both compounds induce DNA damage involving both pyrimidine and purine-based oxidations, at least in CHO-K1 cells. Our findings confirm the genotoxic potential of IMZT and suggest that this herbicide formulation must be employed with great caution, especially not only for exposed occupational workers but also for other living species.