Artículos de revistas
Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects
Fecha
2013-08-15Registro en:
Busto, Víctor Daniel; Calabró López, Roberto Ariel; Rodriguez Talou, Julian; Giulietti, Ana Maria; Merchuk, José C.; Anthraquinones production in Rubia tinctorum cell suspension cultures: Down scale of shear effects; Elsevier; Biochemical Engineering Journal; 77; 15-8-2013; 119-128
1369-703X
Autor
Busto, Víctor Daniel
Calabró López, Roberto Ariel
Rodriguez Talou, Julian
Giulietti, Ana Maria
Merchuk, José C.
Resumen
The effect of turbulence on suspended cells is one of the most complex problems in the scale-up of cell cultures. In the present paper, a direct comparison of the effects of turbulence on suspension cultures of Rubia tinctorum in a standard bioreactor and in shake flask cultures was done. A procedure derived from the well known global method proposed by Nishikawa et al. (1977) was applied. Standard flasks and four-baffled shake flasks were used. The effect of turbulence and light irradiation on cell viability, biomass, and anthraquinones (AQs) production was evaluated. The biomass concentration and AQs production obtained using baffled shake flasks agitated at 360 rpm were similar to that achieved in R. tinctorum suspension cultures growing in a stirred tank bioreactor operating at 450 rpm, previously published (Busto et al., 2008). The effect of light on AQs production was found to be very significant, and a difference of up to 48% was found in cells with and without illumination after 7 days of culture. It is concluded that this down-scaled and simple flask culture system is a suitable and valid small scale instrument for the study of intracellular mechanisms of turbulence-induced AQs production in R. tinctorum suspension cultures.